Omega-based mini prime- and base-editing systems in Escherichia coli

Obligate mobile element-guided activity-based prime-editing and base-editing systems represent a significant advancement in gene editing and targeted mutagenesis, enabling precise, programmable mutations in target genes. These compact, high-efficiency systems seamlessly integrate with multiple high-throughput screening technologies, significantly accelerating directed evolution processes and offering powerful tools for genetic engineering and biotechnological applications.