Highly efficient prime editors for mammalian genome editing based on porcine retrovirus reverse transcriptase

This study developed a highly efficient prime editing system (pvPE) based on porcine endogenous retroviral reverse transcriptase. Through extensive engineering and nocodazole treatment, pvPE achieved robust multigene editing in porcine fibroblasts, which generated Alzheimer’s disease pig models, offering a versatile platform for agricultural and biomedical applications.