Genome-scale CRISPRi and base-editing libraries for genetic decoding and strain engineering in Shewanella

Shewanella oneidensis, an electroactive microbe, was equipped with genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) interference and base-editing libraries to systematically link genes to phenotypes. These tools revealed candidate essential genes and expanded substrate utilization for electricity generation, providing a versatile framework for functional genomics and microbial engineering in bioelectrochemical applications.